The Sm-like Hfq protein increases OxyS RNA interaction with target mRNAs

被引:410
作者
Zhang, AX
Wassarman, KM
Ortega, J
Steven, AC
Storz, G [1 ]
机构
[1] NICHHD, Cell Biol & Metab Branch, NIH, Bethesda, MD 20892 USA
[2] NIAMSD, Struct Biol Lab, NIH, Bethesda, MD 20892 USA
关键词
D O I
10.1016/S1097-2765(01)00437-3
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The Escherichia coli host factor I, Hfq, binds to many small regulatory RNAs and is required for OxyS RNA repression of fhIA and rpoS mRNA translation. Here we report that Hfq is a bacterial homolog of the Sm and Sm-like proteins integral to RNA processing and mRNA degradation complexes in eukaryotic cells. Hfq exhibits the hallmark features of Sm and Sm-like proteins: the Sm1 sequence motif, a multisubunit ring structure (in this case a homomeric hexamer), and preferential binding to polyU. We also show that Hfq increases the OxyS RNA interaction with its target messages and propose that the enhancement of RNA-RNA pairing may be a general function of Hfq, Sm, and Sm-like proteins.
引用
收藏
页码:11 / 22
页数:12
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共 53 条
  • [1] A doughnut-shaped heteromer of human Sm-like proteins binds to the 3′-end of U6 snRNA, thereby facilitating U4/U6 duplex formation in vitro
    Achsel, T
    Brahms, H
    Kastner, B
    Bachi, A
    Wilm, M
    Lührmann, R
    [J]. EMBO JOURNAL, 1999, 18 (20) : 5789 - 5802
  • [2] The Sm domain is an ancient RNA-binding motif with oligo(U) specificity
    Achsel, T
    Stark, H
    Lührmann, R
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (07) : 3685 - 3689
  • [3] The Escherichia coli OxyS regulatory RNA represses fhlA translation by blocking ribosome binding
    Altuvia, S
    Zhang, AX
    Argaman, L
    Tiwari, A
    Storz, G
    [J]. EMBO JOURNAL, 1998, 17 (20) : 6069 - 6075
  • [4] A small, stable RNA induced by oxidative stress: Role as a pleiotropic regulator and antimutator
    Altuvia, S
    WeinsteinFischer, D
    Zhang, AX
    Postow, L
    Storz, G
    [J]. CELL, 1997, 90 (01) : 43 - 53
  • [5] fhlA repression by OxyS RNA:: Kissing complex formation at two sites results in a stable antisense-target RNA complex
    Argaman, L
    Altuvia, S
    [J]. JOURNAL OF MOLECULAR BIOLOGY, 2000, 300 (05) : 1101 - 1112
  • [6] Two types of localization of the DNA-binding proteins within the Escherichia coli nucleoid
    Azam, TA
    Hiraga, S
    Ishihama, A
    [J]. GENES TO CELLS, 2000, 5 (08) : 613 - 626
  • [7] RNA REPLICATION - FUNCTION AND STRUCTURE OF QBETA-REPLICASE
    BLUMENTHAL, T
    CARMICHAEL, GG
    [J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1979, 48 : 525 - 548
  • [8] Mutations that increase expression of the rpoS gene and decrease its dependence on hfq function in Salmonella typhimurium
    Brown, L
    Elliott, T
    [J]. JOURNAL OF BACTERIOLOGY, 1997, 179 (03) : 656 - 662
  • [9] Efficient translation of the RpoS sigma factor in Salmonella typhimurium requires host factor I, an RNA-binding protein encoded by the hfq gene
    Brown, L
    Elliott, T
    [J]. JOURNAL OF BACTERIOLOGY, 1996, 178 (13) : 3763 - 3770
  • [10] CARMICHAEL GG, 1975, J BIOL CHEM, V250, P3607