Environmental effects on the growth and proteolysis of Treponema denticola ATCC 33520

被引:14
作者
Mikx, FHM
机构
[1] Sect. of Prev. and Curative Dent., Subfaculty of Dentistry, University of Nijmegen
[2] Subfaculty of Dentistry, University of Nijmegen, NL-6500 HB, Nijmegen
来源
ORAL MICROBIOLOGY AND IMMUNOLOGY | 1997年 / 12卷 / 04期
关键词
Treponema denticola; redox; pH; oxygen; hydrogen; proteolysis;
D O I
10.1111/j.1399-302X.1997.tb00387.x
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
The effect of pH, redox potential, O-2 and H-2 On the growth and proteolytic activity of Treponema denticola ATCC 33520 was studied in a chemostat at different growth rates. The peptidase and protease activities were estimated using different amido-methyl coumarin derivatives and azocasein. The maximum growth rate of T. denticola ATCC 33520 was 0.14 h(-1). Reduction of the growth rate of T. denticola by 50-60% gave: an increase in cell mass of 150-200%, a higher acetogenesis and a shift of the pH optimum. The protease and phenylalanine peptidase activities seemed to be of greater importance for the growth of T. denticola ATCC 33520 than the rather low arginine and proline peptidase activities. The redox potential (E-h) played a secondary role. At microaerophilic conditions with 1-5% O-2, the cultures maintained a redox potential below -311 mV and an optimal acetogenesis. The presence of H-2 induced a marked growth stimulation of T. denticola ATCC 33520. It is concluded that the cell mass and proteolytic activity of T. denticola ATCC 33520 are modulated by the growth rate and the pH and to a lesser extend by the redox potential and presence of O-2. Stagnation of the exudate-flow influences these factors and will lead to an increase of the spirochetal population and proteolysis in the periodontal pocket.
引用
收藏
页码:249 / 253
页数:5
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