A cDNA clone from Arabidopsis thaliana encoding plastidic ferredoxin:sulfite reductase

被引：31

作者：

Bruhl, A ^{[1
]}

Haverkamp, T ^{[1
]}

Gisselmann, G ^{[1
]}

Schwenn, JD ^{[1
]}

机构：

[1] RUHR UNIV BOCHUM,FAC BIOL,D-44780 BOCHUM,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1996年 / 1295卷 / 02期

关键词：

cysteine biosynthesis; assimilatory sulfite reduction; hydrogen-sulfide:ferredoxin oxidoreductase; cDNA; sequence homology; chloroplast isoform; (A-thaliana);

D O I：

10.1016/0167-4838(96)00066-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A cDNA with an open reading frame of 1929 bp (termed sir) was isolated from a lambda ZapII library of Arabidopsis thaliana leaf tissue. The polypeptide sequence deduced from the cDNA is homologous to the ferredoxin-dependent sulfite reductase (EC 1.8.7.1) from Synechococcus PCC7942 and distantly related to the hemoprotein subunit of Escherichia coli NADPH-dependent sulfite reductase (EC 1.8.1.2). A molecular mass of 71.98 kDa can be predicted for a ferredoxin sulfite reductase from A. thaliana. The polypeptide consists of 642 amino acids including a transit peptide of 66 residues (6.72 kDa) that is assumed to direct the protein into the plastid. For expression and enzymatic characterization of a putative A. thaliana ferredoxin sulfite reductase, the DNA of the transit peptide was deleted by a PCR method. The truncated cDNA clone was expressed as his-tag fusion protein. The modified gene product was enzymatically inactive but specific cross-reaction with polyclonal antibodies against ferredoxin sulfite reductase from Synechococcus is seen as confirmation of its identity as higher plant ferredoxin sulfite reductase.

引用

页码：119 / 124

页数：6

共 31 条

[1] STUDIES OF SULFATE UTILIZATION BY ALGAE .11. ENZYME-BOUND INTERMEDIATE IN REDUCTION OF ADENOSINE-5'-PHOSPHOSULFATE (APS) BY CELL-FREE-EXTRACTS OF WILD-TYPE CHLORELLA AND MUTANTS BLOCKED FOR SULFATE REDUCTION [J].