Separation of phospholipid classes in sea urchin, Paracentrotus lividus by high-performance liquid chromatography

被引：25

作者：

de Quiros, ARB ^{[1
]}

López-Hernández, J ^{[1
]}

Simal-Lozano, J ^{[1
]}

机构：

[1] Univ Santiago Compostela, Nutr Bromatol Grp, Dept Analyt Chem Nutr & Bromatol, Fac Pharm, E-15782 Santiago De Compostela, La Coruna, Spain

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2002年 / 770卷 / 1-2期

关键词：

Paracentrotus lividus; phospholipids;

D O I：

10.1016/S1570-0232(01)00628-6

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

A simple high-performance liquid chromatography (HPLC) method for determination of major phospholipid classes in sea urchin Paracentrotus lividus is described. The separation was performed on a Tracer Extrasil SI 5 mum 25x0.4 cm column and an isocratic mobile phase of acetonitrile-methanol 85%-phosphoric acid (50:50:1.8, v/v). The HPLC method utilizes UV detection at 205 nm. Five phospholipids were identified and quantified: phosphatidylinositol (PI), phosphatidylserine (PS), phosphatidylethanolamine (PE), phosphatidylcholine (PC) and sphingomyelin (SM). Fresh and canned samples were analyzed. Student's t-test showed no significant difference (Pless than or equal to0.05) between the mean phospholipid contents of raw and canned sea urchin. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：71 / 75

页数：5

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