Comparison of two fluorescence immunoassay methods for the detection of endocrine disrupting chemicals in water

被引：40

作者：

Coille, I ^{[1
]}

Reder, S ^{[1
]}

Bucher, S ^{[1
]}

Gauglitz, G ^{[1
]}

机构：

[1] Univ Tubingen, Inst Phys & Theoret Chem, D-72076 Tubingen, Germany

来源：

BIOMOLECULAR ENGINEERING | 2002年 / 18卷 / 06期

关键词：

immunoassays; estrogens; fluorescence; waste water; TIRF; FRET;

D O I：

10.1016/S1389-0344(01)00111-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe two fluorescence immunoassays capable of detecting endocrine disrupting compounds in waste water. The first fluorescence method is a heterogeneous assay using total internal reflection fluorescence (TIRF) detection. The second method is a homogeneous assay that utilizes energy transfer (ETIA). Both fluorescence immunoassays are compared with respect to detection principle and ability to quantify the model analytes estrone, estradiol, and ethinylestradiol in a complex matrix regarding recovery rates and limits of detection. Calibrations were performed for the three analytes using both fluorescence methods. Limits of detection between 0.01 and 0.85 mug/l are achieved. In addition, measurements in synthetic waste water spiked with the analytes were performed. Both immunoassays allow the detection in waste water with recovery rates in the range of 70-112%. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：273 / 280

页数：8

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