Roles of RhoA and phospholipase A2 in the elevation of intracellular H2O2 by transforming growth factor-β in Swiss 3T3 fibroblasts

被引：24

作者：

Koo, HY

Shin, I

Lee, ZW

Lee, SH

Kim, SH

Lee, CH

Kang, HS

Ha, KS

机构：

[1] Korea Basic Sci Inst, Biomol Res Grp, Taejon 305333, South Korea

[2] Kosin Univ, Sch Med, Dept Surg, Pusan 602702, South Korea

[3] Inje Univ, Pusan Paik Hosp, Dept Surg, Pusan 633165, South Korea

[4] Kangnung Univ, Dept Biol, Kangwon Do 210702, South Korea

[5] Pusan Natl Univ, Dept Mol Biol, Pusan 609735, South Korea

来源：

CELLULAR SIGNALLING | 1999年 / 11卷 / 09期

关键词：

transforming growth factor-beta; intracellular H2O2; RhoA; phospholipase A(2); arachidonic acid; stress fibres; DNA synthesis;

D O I：

10.1016/S0898-6568(99)00038-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have investigated the mechanisms by which transforming growth factor-beta(TGF-beta) increased intracellular H2O2 in Swiss 3T3 fibroblasts. Increase of intracellular H2O2 by TGF-beta was maximal at 30 min and blocked by catalase from Aspergillus niger. Scrape-loading of C3 transferase, which down-regulated RhoA, inhibited the production of H2O2 in response to TGF-beta. TGF-beta stimulated release of arachidonic acid, which was completely inhibited by mepacrine, a phospholipase Az inhibitor. Mepacrine also blocked the increase of H2O2 by TGF-beta. In addition, arachidonic acid increased intracellular H2O2. Furthermore, TGF-beta stimulated stress fibre formation, which was blocked by catalase, without membrane ruffling. Catalase also inhibited stimulation of thymidine incorporation by TGF-beta. These results suggested that TGF-beta increased intracellular H2O2 through RhoA and phospholipase A(2), and also suggested that intracellular H2O2 was required for the stimulation of stress fibre formation and DNA synthesis in response to TGF-beta. (C) 1999 Elsevier Science Inc.

引用

页码：677 / 683

页数：7

共 36 条

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