Amino-acid limitation induces the GCN2 signaling pathway in myoblasts but not in myotubes

被引：8

作者：

Deval, Christiane ^{[1
]}

Talvas, Jeremie ^{[1
]}

Chaveroux, Cedric ^{[1
]}

Maurin, Anne-Catherine ^{[1
]}

Mordier, Sylvie ^{[1
]}

Cherasse, Yoan ^{[1
]}

Parry, Laurent ^{[1
]}

Carraro, Valrie ^{[1
]}

Jousse, Celine ^{[1
]}

Bruhat, Alain ^{[1
]}

Fafournoux, Pierre ^{[1
]}

机构：

[1] INRA, Unite Nutr Humaine, UMR 1019, F-63122 St Genes Champanelle, France

来源：

BIOCHIMIE | 2008年 / 90卷 / 11-12期

关键词：

Amino acid; Gene expression; GCN2; Myotube; Myoblast;

D O I：

10.1016/j.biochi.2008.07.004

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

There is a growing body of evidence that suggests that amino acids play an important role in controlling gene expression, but the cell specificity of the amino-acid-mediated regulation of gene expression in mammals remains unknown. Using a model of muscle cells (C2C12) at two stages of differentiation, i.e. myoblasts and myotubes, we employed transcriptional profiling to show that amino-acid deficiency does not regulate the same set of gene in differentiated and non-differentiated cells. Furthermore, in myotubes, the GCN2 pathway is not activated by amino-acid starvation due to an amino-acid supply from intracellular protcolysis associated with a low GCN2 expression. (c) 2008 Elsevier Masson SAS. All rights reserved.

引用

页码：1716 / 1721

页数：6

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