Nanoassembly of biodegradable microcapsules for DNA encasing

被引：156

作者：

Shchukin, DG

Patel, AA

Sukhorukov, GB

Lvov, YM ^{[1
]}

机构：

[1] Louisiana Tech Univ, Inst Micromfg, Ruston, LA 71272 USA

[2] Max Planck Inst Colloids & Interfaces, D-14424 Potsdam, Germany

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2004年 / 126卷 / 11期

关键词：

D O I：

10.1021/ja036952x

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A new microcontainer for DNA delivery based on biocompatible poly[β-glucuronic acid-(1 → 3)-N-acetyl-β-galactosamine-6-sulfate-(1 → 4)](chondroitin sulfate)/poly(-l-arginine) microcapsules with 40 nm thick molecularly organized shell was proposed. DNA molecules were deposited as DNA/sperimidine complex on the surface of template 4 μm core particles followed by layer-by-layer nanoassembly of protective chondroitin sulfate/poly(-l-arginine) shell. After template core dissolution, DNA molecules were captured inside microcapsules retaining a natural double-helix structure. The developed DNA encapsulation approach can be employed for targeted delivery of plasmid DNA in living cells. Copyright © 2004 American Chemical Society.

引用

页码：3374 / 3375

页数：2

共 27 条

[1] Efficient encapsulation of DNA plasmids in small neutral liposomes induced by ethanol and calcium [J].