A Cre/loxP-deleter transgenic line in mouse strain 129S1/SvImJ

被引：140

作者：

Tang, SHE ^{[1
]}

Silva, FJ ^{[1
]}

Tsark, WMK ^{[1
]}

Mann, JR ^{[1
]}

机构：

[1] City Hope Natl Med Ctr, Beckman Res Inst, Div Biol, Duarte, CA 91010 USA

来源：

GENESIS | 2002年 / 32卷 / 03期

关键词：

gene targeting; deleter; transgene; Cre recombinase; Hprt;

D O I：

10.1002/gene.10030

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A Cre recombinase expression cassette was inserted into the X-linked Hprt locus by gene targeting in a mouse embryonic stem (ES) cell line isogenic to strain 129S1/SvImJ (129S1), then the transgene was introduced into 129S1 mice through ES cell chimeras. When females hemizygous for this transgene were mated to males carrying a neomycin selection cassette flanked by loxP sites, the cassette was always excised regardless of Cre inheritance and without detectable mosaicism. The usefulness of this "Cre-deleter" transgenic line is in its efficiency and defined genetic status in terms of mouse strain and location of the transgene. genesis 32: 199-202, 2002. (C) 2002 Wiley-Liss, Inc.

引用

页码：199 / 202

页数：4

共 22 条

[1] Single-copy transgenic mice with chosen-site integration [J].

Bronson, SK ;

Plaehn, EG ;

Kluckman, KD ;

Hagaman, JR ;

Maeda, N ;

Smithies, O .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (17) :9067-9072

[2] Appropriate tissue- and cell-specific expression of a single copy human angiotensinogen transgene specifically targeted upstream of the HPRT locus by homologous recombination [J].

Cvetkovic, B ;

Yang, B ;

Williamson, RA ;

Sigmund, CD .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2000, 275 (02) :1073-1078

[3] REEXAMINATION OF GENE TARGETING FREQUENCY AS A FUNCTION OF THE EXTENT OF HOMOLOGY BETWEEN THE TARGETING VECTOR AND THE TARGET LOCUS [J].

DENG, CX ;

CAPECCHI, MR .

MOLECULAR AND CELLULAR BIOLOGY, 1992, 12 (08) :3365-3371

[4] Targeting the Hprt locus in mice reveals differential regulation of Tie2 gene expression in the endothelium [J].

Evans, V ;

Hatzopoulos, A ;

Aird, WC ;

Rayburn, HB ;

Rosenberg, RD ;

Kuivenhoven, JA .

PHYSIOLOGICAL GENOMICS, 2000, 2 (02) :67-75

[5]

FESTING MFW, 2001, MOUSE GENOME INFORMA

[6] Targeting of human eNOS promoter to the Hprt locus of mice leads to tissue-restricted transgene expression [J].

Guillot, PV ;

Liu, LX ;

Kuivenhoven, JA ;

Guan, J ;

Rosenberg, RD ;

Aird, WC .

PHYSIOLOGICAL GENOMICS, 2000, 2 (02) :77-83

[7] The influence of chromosomal location on the expression of two transgenes in mice [J].

Hatada, S ;

Kuziel, W ;

Smithies, O ;

Maeda, N .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (02) :948-955

[8]

Hogan BLM, 1993, MANIPULATING MOUSE E

[9] LOSS OF THE IMPRINTED IGF2/CATION-INDEPENDENT MANNOSE 6-PHOSPHATE RECEPTOR RESULTS IN FETAL OVERGROWTH AND PERINATAL LETHALITY [J].

LAU, MMH ;

STEWART, CEH ;

LIU, ZY ;

BHATT, H ;

ROTWEIN, P ;

STEWART, CL .

GENES & DEVELOPMENT, 1994, 8 (24) :2953-2963

[10] A transgenic mouse line that retains Cre recombinase activity in mature oocytes irrespective of the cre transgene transmission [J].

Sakai, K ;

Miyazaki, J .

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1997, 237 (02) :318-324

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