Dissemination and Transmission of the E1-226V Variant of Chikungunya Virus in Aedes albopictus Are Controlled at the Midgut Barrier Level

被引:60
作者
Arias-Goeta, Camilo [1 ,2 ]
Mousson, Laurence [1 ]
Rougeon, Francois [3 ]
Failloux, Anna-Bella [1 ]
机构
[1] Inst Pasteur, Dept Virol Arboviruses & Insect Vectors, Paris, France
[2] Univ Paris 06, Cellule Pasteur, Paris, France
[3] Inst Pasteur, CNRS, URA 2581, Paris, France
来源
PLOS ONE | 2013年 / 8卷 / 02期
关键词
MOSQUITO; VECTORS; ARBOVIRUSES; INFECTION; EMERGENCE; CULICIDAE; EPIDEMIC; OUTBREAK; PATTERNS; DIPTERA;
D O I
10.1371/journal.pone.0057548
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Emergence of arboviruses could result from their ability to exploit new environments, for example a new host. This ability is facilitated by the high mutation rate occurring during viral genome replication. The last emergence of chikungunya in the Indian Ocean region corroborates this statement since a single viral mutation at the position 226 on the E1 glycoprotein (E1-A226V) was associated with enhanced transmission by the mosquito Aedes albopictus in regions where the major mosquito vector, Aedes aegypti, is absent. We used direct competition assays in vivo to dissect out the mechanisms underlying the selection of E1-226V by Ae. albopictus. When the original variant E1-226A and the newly emerged E1-226V were provided in the same blood-meal at equal titers to both species of mosquitoes, we found that the proportion of both variants was drastically different in the two mosquito species. Following ingestion of the infectious blood-meal, the E1-226V variant was preferentially selected in Ae. albopictus, whereas the E1-226A variant was sometimes favored in Ae. aegypti. Interestingly, when the two variants were introduced into the mosquitoes by intrathoracic inoculations, E1-226V was no longer favored for dissemination and transmission in Ae. albopictus, showing that the midgut barrier plays a key role in E1-226V selection. This study sheds light on the role of the midgut barrier in the selection of novel arbovirus emerging variants. We also bring new insight into how the pre-existing variant E1-226V was selected among other viral variants including E1-226A. Indeed the E1-226V variant present at low levels in natural viral populations could rapidly emerge after being selected in Ae. albopictus at the midgut barrier level.
引用
收藏
页数:9
相关论文
共 27 条
[1]   Genetic divergence of Chikungunya viruses in India (1963-2006) with special reference to the 2005-2006 explosive epidemic [J].
Arankalle, Vidya A. ;
Shrivastava, Shubham ;
Cherian, Sarah ;
Gunjikar, Rashmi S. ;
Walimbe, Atul M. ;
Jadhav, Santosh M. ;
Sudeep, A. B. ;
Mishra, Akhilesh C. .
JOURNAL OF GENERAL VIROLOGY, 2007, 88 :1967-1976
[2]   Progressive Decrease in Aedes aegypti Distribution in Reunion Island Since the 1900s [J].
Bagny, Leila ;
Delatte, Helene ;
Quilici, Serge ;
Fontenille, Didier .
JOURNAL OF MEDICAL ENTOMOLOGY, 2009, 46 (06) :1541-1545
[3]   Aedes (Diptera: Culicidae) Vectors of Arboviruses in Mayotte (Indian Ocean): Distribution Area and Larval Habitats [J].
Bagny, Leila ;
Delatte, Helene ;
Elissa, Nohal ;
Quilici, Serge ;
Fontenille, Didier .
JOURNAL OF MEDICAL ENTOMOLOGY, 2009, 46 (02) :198-207
[4]   The Chikungunya threat: an ecological and evolutionary perspective [J].
Chevillon, Christine ;
Briant, Laurence ;
Renaud, Francois ;
Devaux, Christian .
TRENDS IN MICROBIOLOGY, 2008, 16 (02) :80-88
[5]   Drought-associated Chikungunya emergence along coastal East Africa [J].
Chretien, Jean-Paul ;
Anyamba, Assaf ;
Bedno, Sheryl A. ;
Breiman, Robert F. ;
Sang, Rosemary ;
Sergon, Kibet ;
Powers, Ann M. ;
Onyango, Clayton O. ;
Small, Jennifer ;
Tucker, Compton J. ;
Linthicum, Kenneth J. .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2007, 76 (03) :405-407
[6]   Chikungunya virus adapts to tiger mosquito via evolutionary convergence: a sign of things to come? [J].
de Lamballerie, Xavier ;
Leroy, Eric ;
Charrel, Remi N. ;
Ttsetsarkin, Konstantin ;
Higgs, Stephen ;
Gould, Ernest A. .
VIROLOGY JOURNAL, 2008, 5 (1)
[7]   Geographic distribution and developmental sites of Aedes albopictus (Diptera: Culicidae) during a Chikungunya epidemic event [J].
Delatte, H. ;
Dehecq, J. S. ;
Thiria, J. ;
Domerg, C. ;
Paupy, C. ;
Fontenille, D. .
VECTOR-BORNE AND ZOONOTIC DISEASES, 2008, 8 (01) :25-34
[8]   Vectors of Chikungunya virus in Senegal: Current data and transmission cycles [J].
Diallo, M ;
Thonnon, J ;
Traore-Lamizana, M ;
Fontenille, D .
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 1999, 60 (02) :281-286
[9]   Chikungunya Virus and Aedes Mosquitoes: Saliva Is Infectious as soon as Two Days after Oral Infection [J].
Dubrulle, Mathieu ;
Mousson, Laurence ;
Moutailler, Sara ;
Vazeille, Marie ;
Failloux, Anna-Bella .
PLOS ONE, 2009, 4 (06)
[10]  
JUPP PG, 1990, J AM MOSQUITO CONTR, V6, P415