Elevated cell-associated levels of interleukin 1 beta and interleukin 6 in inflamed mucosa of inflammatory bowel disease

被引:32
作者
GrottrupWolfers, E
Moeller, J
Karbach, U
MullerLissner, S
Endres, S
机构
[1] UNIV MUNICH,KLINIKUM INNENSTADT,MED KLIN,D-80336 MUNICH,GERMANY
[2] HUMBOLDT UNIV BERLIN,VIRCHOW KLINIKUM,BERLIN,GERMANY
[3] STADT KRANKENHAUS BERLIN WEISSENSEE,BERLIN,GERMANY
关键词
inflammatory bowel disease; interleukin; 1; 6; tumour necrosis factor;
D O I
10.1046/j.1365-2362.1996.123262.x
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
The aim of this study was to investigate the involvement of the monocyte-derived cytokines interleukin 1 beta (IL-1 beta), interleukin 6 (IL-6) and tumour necrosis factor-alpha (TNF-alpha) in idiopathic inflammatory bowel disease. Endoscopic biopsies of normal and inflamed intestinal mucosa were obtained from patients with ulcerative colitis (n = 11) and with Crohn's disease (n = 10). Intestinal mucosal cells were isolated by collagenase digestion. Cell viability, morphology and CD14 expression were determined. To measure cell-associated cytokine levels, cells were lysed and analysed for IL-1 beta and TNF-alpha in specific radioimmunoassays and for IL-6 using a biological assay. Compared with mucosal cells from control patients without inflammatory bowel disease the inflamed intestine in ulcerative colitis and Crohn's disease displayed markedly enhanced levels of IL-I beta (median 245pg 10(-6) cells, range 30-1275) and IL-6 (median 22 U 10(-6) cells, range 1-298). Non-inflamed mucosa in patients with ulcerative colitis and Crohn's disease did not shown elevated levels of lL-1 beta (median 50pg 10(-6) cells, range 33-90) or IL-6 (mean below detection limit of assay, i.e. 1 U 10(-6) cells). In contrast, no clear cut difference between inflamed and non-inflamed mucosa could be detected for TNF-alpha. High tissue levels of IL-6 were associated with a high endoscopic grade of local inflammation. These results suggest that the monocyte-derived cytokines IL-1 beta and IL-6 are mediators of inflammation in inflammatory bowel disease.
引用
收藏
页码:115 / 122
页数:8
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