Biosynthesis of [1-15N] l-tryptophan from 15N labeled anthranilic acid by fermentation of Candida utilis mutant

被引：11

作者：

Liu, Zhanfeng ^{[1
]}

Yuan, Qipeng ^{[1
]}

Wang, Wenchuan ^{[2
]}

机构：

[1] Beijing Inst Chem Technol, Coll Life Sci & Technol, Beijing 100029, Peoples R China

[2] Beijing Inst Chem Technol, Coll Chem Engn, Beijing 100029, Peoples R China

来源：

AMINO ACIDS | 2009年 / 36卷 / 01期

关键词：

1-N-15] L-Tryptophan; N-15] Anthranilic acid; Candida utilis mutant;

D O I：

10.1007/s00726-008-0030-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

A new method for synthesizing the labeled l-tryptophan is described in this work. <l-Tryptophan, labeled with 98% N-15 at position 1 was synthesized from the labeled anthranilic acid using Candida utilis mutants. The conversion ratio of N-15 of 50% was achieved. The labeled anthranilic acid was synthesized by [N-15] phthalimide that was prepared by 99.34% [N-15] urea and phthalic anhydride in ortho-xylene medium at 140A degrees C and under atmospheric pressure.

引用

页码：71 / 73

页数：3

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