Prostaglandins stimulate renin secretion and renin mRNA in mouse renal juxtaglomerular cells

This study examined 1) effects of prostaglandins (PG) on renin secretion and renin gene expression from isolated juxtaglomerular granular cells and 2) expression of cyclooxygenases in juxtaglomerular structures. Incubation of granular cell cultures with PGE(2), -I-2, -F2(alpha), and thromboxane B-2 identified PGI(2) and PGE(2) as stimulators of renin secretion; the effects were dose and time dependent. PGE(2) also increased renin mRNA accumulation time and dose dependent. PGE(2) and PGI(2) activated adenylate cyclase concentration dependent in granular cells. PGE(2) stimulations of renin secretion and renin mRNA were nonadditive to those of forskolin and were inhibited by endothelin. The findings are compatible with cellular actions through adenosine 3',5'-cyclic monophosphate (cAMP). On total RNA harvested from whole kidneys, from microdissected glomeruli with attached afferent arterioles and from mesangial cells in primary culture, reverse transcription-polymerase chain reaction revealed significant expression of cyclooxygenase I and II. By direct interaction with PG receptors on renal juxtaglomerular cells, PGE(2) and PGI(2) can act as potent and rapid stimulators of renin secretion and renin mRNA probably through cAMP-dependent pathways.