Physicochemical analysis of structural changes in DNA modified with glucose

被引：28

作者：

Ashraf, Jalaluddin M. ^{[1
]}

Arif, Binish ^{[1
]}

Dixit, Kiran ^{[1
]}

Moinuddin ^{[1
]}

Alam, Khursheed ^{[1
]}

机构：

[1] Aligarh Muslim Univ, Dept Biochem, Fac Med, Aligarh 202002, Uttar Pradesh, India

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2012年 / 51卷 / 04期

关键词：

DNA Amadori; DNA advanced glycation end products; CEdG; GLYCATION END-PRODUCTS; L-ASCORBIC-ACID; NONENZYMATIC GLYCATION; ADVANCED GLYCOSYLATION; MAILLARD REACTION; REDUCING SUGARS; GLUCOSE-6-PHOSPHATE; IMMUNOGENICITY; METHYLGLYOXAL; ANTIGENICITY;

D O I：

10.1016/j.ijbiomac.2012.06.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Reactions of reducing sugars with free amino groups of proteins can form advanced glycation end products (AGES). While the formation of nucleoside AGEs has been studied in detail, no extensive work has been carried out to assess DNA Amadori and DNA advanced glycation end products. In this study, we report biophysical/chemical characterization of glucose-induced changes in DNA, as well as DNA Amadori and DNA advanced glycation end products. Glucose treated DNA exhibited hyperchromicity, decrease in melting temperature, and enhanced emission intensity in a time dependent manner. Formation of DNA Amadori product and DNA advanced glycation end products, mainly CEdG (N-2-carboxyethyl-2'-deoxyguanosine), were the major outcome of the study. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：604 / 611

页数：8

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