Evaluation of Anti-A/Udorn/307/1972 Antibody Specificity to Influenza A/H3N2 Viruses Using an Evanescent-Field Coupled Waveguide-Mode Sensor

被引:42
作者
Gopinath, Subash C. B. [1 ]
Awazu, Koichi [1 ]
Fujimaki, Makoto [1 ]
Shimizu, Kazufumi [2 ]
机构
[1] Natl Inst Adv Ind Sci & Technol, Elect & Photon Res Inst, Tsukuba, Ibaraki, Japan
[2] Nihon Univ, Sch Med, Open Res Ctr Genome & Infect Dis Control, Itabashi Ku, Tokyo, Japan
来源
PLOS ONE | 2013年 / 8卷 / 12期
关键词
A VIRUS; RNA APTAMER; HEMAGGLUTININ; SENSITIVITY; PRINCIPLES; CONSTANTS;
D O I
10.1371/journal.pone.0081396
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Discrimination of closely related strains is a key issue, particularly for infectious diseases whose incidence fluctuates according to variations in the season and evolutionary changes. Among infectious diseases, influenza viral infections are a worldwide cause of pandemic disease and mortality. With the emergence of different influenza strains, it is vital to develop a method using antibodies that can differentiate between viral types and subtypes. Ideally, such a system would also be user friendly. In this study, a polyclonal antibody generated against A/Udorn/307/1972 (H3N2) was used as a probe to distinguish between influenza H3N2 viruses based on the interaction between the antibody and hemagglutinin, demonstrating its applicability for viral discrimination. Clear discrimination was demonstrated using an evanescent-field-coupled waveguide-mode sensor, which has appealing characteristics over other methods in the viewpoint of improving the sensitivity, measurement time, portability and usability. Further supporting evidence was obtained using enzyme-linked immunosorbent assays, hemagglutination-inhibition assays, and infectivity neutralization assays. The results obtained indicate that the polyclonal antibody used here is a potential probe for distinguishing influenza viruses and, with the aid of a handheld sensor it could be used for influenza surveillance.
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页数:9
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