In-vitro analysis of APA microcapsules for oral delivery of live bacterial cells

被引:31
作者
Chen, H [1 ]
Ouyang, W [1 ]
Jones, M [1 ]
Haque, T [1 ]
Lawuyi, B [1 ]
Prakash, S [1 ]
机构
[1] McGill Univ, Biomed Technol Cell Therapy Res Lab, Dept Biomed Engn Physiol, Artificial Cells Organs Res Ctr, Montreal, PQ H3A 2B4, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
alginate; artificial cells; live bacteria; microcapsules; oral delivery;
D O I
10.1080/02652040500162162
中图分类号
O69 [应用化学];
学科分类号
081704 ;
摘要
Oral administration of microcapsules containing live bacterial cells has potential as an alternative therapy for several diseases. This article evaluates the suitability of the alginate-poly-L-lysine-alginate (APA) microcapsules for oral delivery of live bacterial cells, in-vitro, using a dynamic simulated human gastro-intestinal (GI) model. Results showed that the APA microcapsules were morphologically stable in the simulated stomach conditions, but did not retain their structural integrity after a 3-day exposure in simulated human GI media. The microbial populations of the tested bacterial cells and the activities of the tested enzymes in the simulated human GI suspension were not substantially altered by the presence of the APA microcapsules, suggesting that there were no significant adverse effects of oral administration of the APA microcapsules on the flora of the human gastrointestinal tract. When the APA microcapsules containing Lactobacillus plantarum 80 (LP80) were challenged in the simulated gastric medium (pH = 2.0), 80.0% of the encapsulated cells remained viable after a 5-min incubation; however, the viability decreased considerably (8.3%) after 15 min and dropped to 2.6% after 30 min and lower than 0.2% after 60 min, indicating the limitations of the currently obtainable APA membrane for oral delivery of live bacteria. Further in-vivo studies are required before conclusions can be made concerning the inadequacy of APA microcapsules for oral delivery of live bacterial cells.
引用
收藏
页码:539 / 547
页数:9
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