Compositional characterisation of soluble apple polysaccharides, and their antioxidant and hepatoprotective effects on acute CCl4-caused liver damage in mice

被引：109

作者：

Yang, Xingbin ^{[1
]}

Yang, Su ^{[1
]}

Guo, Yurong ^{[1
]}

Jiao, Yadong ^{[1
]}

Zhao, Yan ^{[2
]}

机构：

[1] Shaanxi Normal Univ, Coll Food Engn & Nutr Sci, Minist Educ Med Resource & Nat Pharmaceut Chem, Key Lab, Xian 710062, Peoples R China

[2] Fourth Mil Med Univ, Sch Pharm, Xian 710032, Peoples R China

来源：

FOOD CHEMISTRY | 2013年 / 138卷 / 2-3期

基金：

中国国家自然科学基金;

关键词：

Apple polysaccharides; Peel and flesh; HPLC; Antioxidant activity; Hepatoprotective effect; POLYPHENOL-RICH EXTRACT; CARBON-TETRACHLORIDE; COLORIMETRIC METHOD; HOUTTUYNIA-CORDATA; MOUSE-LIVER; PEELS; RATS; JUICES; INJURY; CCL4;

D O I：

10.1016/j.foodchem.2012.10.030

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Water-soluble apple peel polysaccharides (APP) and apple flesh polysaccharides (AFP) were isolated from Pink Lady fruits, and their in vitro antioxidant capacities were characterised by DPPH., HO., and O-2(-) systems, and ferric-reducing antioxidant power assay. Oral administration of APP at 250 and 500 mg/kg bw in mice was shown to be as effective as AFP in lowering the CCl4-caused increases of serum alanine aminotransferase, aspartate aminotransferase and lactic dehydrogenase activities, and hepatic malondialdehyde level, and antagonising the decreases in antioxidant superoxide dismutase and glutathione peroxidase activities caused by CCl4 (p < 0.05). Histopathological examinations further confirmed that both APP and AFP could protect the liver from CCl4-induced histological alteration. HPLC analysis also showed similar profiles of monosaccharide composition for APP and AFP with arabinose, galactose and galacturonic acid being main component monosaccharides. All of these findings demonstrate that the extracts of both APP and AFP possess antioxidant and hepatoprotective potential. (C) 2012 Elsevier Ltd. All rights reserved.

引用

页码：1256 / 1264

页数：9

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