Determination of cyclophosphamide and its metabolites in human plasma by high-performance liquid chromatography-mass spectrometry

被引：51

作者：

Baumann, F

Lorenz, C

Jaehde, U

Preiss, R

机构：

[1] Univ Leipzig, Dept Clin Pharmacol, D-04107 Leipzig, Germany

[2] Free Univ Berlin, Dept Clin Pharm, Inst Pharm 1, D-1000 Berlin, Germany

来源：

JOURNAL OF CHROMATOGRAPHY B | 1999年 / 729卷 / 1-2期

关键词：

cyclophosphamide;

D O I：

10.1016/S0378-4347(99)00178-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A sensitive HPLC-MS method was developed for the simultaneous determination of cyclophosphamide and its metabolites 4-hydroxycyclophosphamide (aldocyclophosphamide), 4-ketocyclophosphamide, caboxyphosphamide and 3-dechloroethylifosfamide in human plasma. 4-Hydroxycyclophosphamide was converted with methylhydroxylamine to the stable methyloxime form. We used a solid-phase extraction with C-18 cartridges followed by HPLC-MS with the single mass spectrometer SSQ 7000 of Finnigan. The limits of detection were 15 ng/ml for cyclophosphamide, 3-dechloroethylifosfamide and ketocyclophosphamide in each case and 30 ng/ml for carboxyphosphamide and 4-hydroxycyclophosphamide, respectively. First results of pharmacokinetics, are shown. (C) 1999 Elsevier Science B.V. All rights reserved.

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页码：297 / 305

页数：9

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