Extracellular keratinase from Trichophyton sp HA-2 isolated from feather dumping soil

被引：39

作者：

Anbu, Periasamy ^{[1
,2
]}

Hilda, Azariah ^{[2
]}

Sur, Hwal-Won ^{[1
]}

Hur, Byung-Ki ^{[1
]}

Jayanthi, S. ^{[1
]}

机构：

[1] Inha Univ, Dept Biol Engn, Inchon 402751, South Korea

[2] Univ Madras, Ctr Adv Studies Bot, Madras 600025, Tamil Nadu, India

来源：

INTERNATIONAL BIODETERIORATION & BIODEGRADATION | 2008年 / 62卷 / 03期

关键词：

Extracellular keratinase; Keratinophilic fungi; Keratin; Purification; Trichophyton species;

D O I：

10.1016/j.ibiod.2007.07.017

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A native feather-degrading keratinophilic fungus, Trichophyton sp. HA-2, was isolated from feather dumping soil in India and screened for extracellular keratinase using keratin-agar medium (chicken feathers as substrate). The fungus was grown in mineral salt medium and keratinase production was tested. The highest keratinase production was observed at pH 8.0 and a temperature of 35 degrees C for a period of 5 weeks. Keratinase secretion was enhanced when cultures were supplemented with a carbon source and keratin source (chicken feathers). This novel keratinase was purified by DEAE-cellulose and sephadex G-100 column chromatography and characterized. The molecular weight of the keratinase was estimated to be 34 kDa by SDS-PAGE. The optimum pH and temperature were determined to be 7.8 and 40 degrees C, respectively. This enzyme was completely inhibited by phenylmethylsulfonyl fluoride (PMSF), indicating the presence of serine protease. (C) 2008 Elsevier Ltd. All rights reserved.

引用

页码：287 / 292

页数：6

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