Cloning and sequencing of the DnaK locus in Streptomyces coelicolor A3(2)

被引：8

作者：

Brans, A ^{[1
]}

Loriaux, A ^{[1
]}

Joris, B ^{[1
]}

Dusart, J ^{[1
]}

机构：

[1] UNIV LIEGE,INST CHIM,CTR INGN PROT,B-4000 LIEGE 1,BELGIUM

来源：

DNA SEQUENCE | 1996年 / 6卷 / 03期

关键词：

DnaJ; chaperones; grpE; heat-shock proteins;

D O I：

10.3109/10425179609010207

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The dnaK operon of Streptomyces coelicolor A3(2) was cloned by the DNA-probing method using synthetic oligonucleotides designed on the basis of two of the most conserved regions in 30 different DnaK proteins (HSP70). The isolated insert-a BamHI 5.6-kb fragment-was sequenced and shown to contain three open-reading frames organized in an operon and coding for proteins analogous to DnaK, GrpE and DnaJ, successively.

引用

页码：179 / 184

页数：6

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