Potency and Fate Specification in CNS Stem Cell Populations In Vitro

被引：87

作者：

Ravin, Rea ^{[1
]}

Hoeppner, Daniel J. ^{[1
]}

Munno, David M. ^{[1
]}

Carmel, Liran ^{[2
]}

Sullivan, Jim ^{[1
]}

Levitt, David L. ^{[1
]}

Miller, Jennifer L. ^{[1
]}

Athaide, Christopher ^{[3
]}

Panchision, David M. ^{[4
]}

Mckay, Ronald D. G. ^{[1
]}

机构：

[1] NINDS, Mol Biol Lab, NIH, Bethesda, MD 20892 USA

[2] Natl Lib Med, NIH, Bethesda, MD 20894 USA

[3] EYE Biomachines, Houston, TX 77005 USA

[4] Childrens Natl Med Ctr, Neurosci Res Ctr, Childrens Res Inst, Washington, DC 20010 USA

来源：

CELL STEM CELL | 2008年 / 3卷 / 06期

关键词：

D O I：

10.1016/j.stem.2008.09.012

中图分类号：

Q813 [细胞工程];

学科分类号：

摘要：

To realize the promise of stem cell biology, it is important to identify the precise time in the history of the cell when developmental potential is restricted. To achieve this goal, we developed a real-time imaging system that captures the transitions in fate, generating neurons, astrocytes, and oligodendrocytes from single CNS stem cells in vitro. In the presence of bFGF, tripotent cells normally produce specified progenitors through a bipotent intermediate cell type. Surprisingly, the tripotent state is reset at each passage. The cytokine CNTF is thought to instruct multipotent cells to an astrocytic fate. We demonstrate that CNTF both directs astrogliogenesis from tripotent cells, bypassing two of the three normal bipotent intermediates, and later promotes the expansion of specified astrocytic progenitors. These results show how discrete cell types emerge from a multipotent cell and provide a strong basis for future studies to determine the molecular basis of fate specification.

引用

页码：670 / 680

页数：11

共 35 条

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