Insertional gene fusion technology

被引：44

作者：

Doi, N ^{[1
]}

Yanagawa, H ^{[1
]}

机构：

[1] Mitsubishi Kasei Inst Life Sci, Machida, Tokyo 1948511, Japan

来源：

FEBS LETTERS | 1999年 / 457卷 / 01期

关键词：

domain insertion; protein engineering; membrane protein topology; directed evolution; biosensor; green fluorescent protein;

D O I：

10.1016/S0014-5793(99)00991-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The classical 'end to end' gene fusion technique has widely been used for monitoring gene expression, biological screening and purification of recombinant proteins. Recent progress with the (insertional) gene fusion approach, on the other hand, has demonstrated that this technique can be utilized for membrane protein topology analysis, display of randomized protein libraries and design of biosensor proteins. In this review, we describe examples of insertional gene fusion and compare the old and new gene fusion techniques. (C) 1999 Federation of European Biochemical Societies.

引用

页码：1 / 4

页数：4

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