A study on growth characteristics and nutrient consumption of Lactobacillus plantarum in A-stat culture

被引：17

作者：

Kask, S ^{[1
]}

Laht, TM ^{[1
]}

Pall, T ^{[1
]}

Paalme, T ^{[1
]}

机构：

[1] Inst Chem Phys & Biophys, EE-12618 Tallinn, Estonia

来源：

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY | 1999年 / 75卷 / 04期

关键词：

amino acid metabolism; A-stat; growth characteristics; Lactobacillus plantarum;

D O I：

10.1023/A:1001868005416

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Lactobacillus plantarum was grown in complex media containing glucose and yeast extract. The maximum growth yield based on yeast extract consumption was 0.5 g dwt g(-1). Growth yield Y-ATP 15-17 g dwt mol ATP(-1) was almost constant in the glucose limited A-stat experiment whereas in the yeast extract limited culture it increased with dilution rate. The maximum specific growth rate observed, 0.5 h(-1), was similar for both A-stat and batch cultures. Specific oxygen consumption, Q(O2), reached the value of 1.8 mmol O-2 h(-1) g dwt(-1). It was shown that Val, Ile, Leu, Tyr and Phe, were consumed mainly as free amino acids, while Asp, Pro, Lys and Arg were derived from peptides. Significantly more Asp, Ser, Glu, Val, Ile, Leu and Phe were consumed than needed to build up cell protein whereas some Pro, Gly, Ala and Lys was synthesized. A network of metabolic reactions in L. plantarum was proposed on the basis of the experimental data.

引用

页码：309 / 320

页数：12

共 27 条

[1] THE EFFECT OF YEAST EXTRACT SUPPLEMENTATION ON THE PRODUCTION OF LACTIC-ACID FROM WHEY PERMEATE BY LACTOBACILLUS-HELVETICUS [J].