Stomatal and non-stomatal limitations of photosynthesis under water stress in field-grown grapevines

Long-term induced water stress in field-grown grapevine leads to a progressive decline of stomatal conductance, accompanied by a decrease in CO2 assimilation (40%). The apparent quantum yield also decreases (59%), which may reflect a relative increase in alternative processes for electron consumption. There is also a shift to non-stomatal regulation, as judged from significant depletions (37%) in maximum photosynthesis rate at saturating CO2 related to limited ribulose biphosphate (RuBP) regeneration, whereas small, non-significant effects are observed on carboxylation efficiency. A high correlation (87%) between photosynthesis and stomatal conductance is observed for all experimental data and declines in intercellular CO2 concentration parallel reductions in stomatal conductance. The data show that field response of grapevines to increasing soil water deficit involves stomatal and non-stomatal effects but, due to gradually induced drought, regulation mechanisms able to adjust mesophyll capacity to the average CO2 supply. The non-stomatal adjustment seems to be exerted mainly in metabolic pathways related with the RuBP regeneration. Contrasting characteristics were observed for both cultivars. Tempranillo exploited the non-stressful conditions successfully, whereas Manto Negro, responding to its reputation as more drought resistant, showed a higher intrinsic water use efficiency, particularly for low water availability. This advantage seems to be due to lower non-stomatal limitations.