Chloroplast SecE:: evidence for spontaneous insertion into the thylakoid membrane

被引：12

作者：

Steiner, JM ^{[1
]}

Köcher, T ^{[1
]}

Nagy, C ^{[1
]}

Löffelhardt, W ^{[1
]}

机构：

[1] Univ Vienna, Inst Biochem & Mol Zellbiol, Ludwig Boltzmann Forsch Stelle Biochem, A-1030 Vienna, Austria

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 293卷 / 02期

基金：

奥地利科学基金会;

关键词：

SecE; Arabidopsis thaliana; preprotein translocase; thylakoid insertion; spinach chloroplasts; spontaneous pathway;

D O I：

10.1016/S0006-291X(02)00285-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SecE, an essential component of the bacterial SecAYEG translocase, mediates protein translocation across the cytoplasmic membrane. In the thylakoid membranes of chloroplasts an SecE homologue, cpSecE, has recently been identified. In this report we show that insertion of cpSecE does not require stromal extract, indicating that signal recognition particle is not involved. Removal of nucleoside triphosphates has apparently no effect on the integration, again ruling out an involvement of SRP or its partner protein, FtsY. The use of well-known inhibitors of the Sec- and Tat pathways, sodium azide and nigericin, respectively, also had no influence on membrane insertion. The data presented here point towards cpSecE as another passenger of a wholly spontaneous import/insertion pathway in the thylakoids of chloroplasts. (C) 2002 Elsevier Science (USA). All rights reserved.

引用

页码：747 / 752

页数：6

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