A checkpoint protein that scans the chromosome for damage at the start of sporulation in Bacillus subtilis

被引:125
作者
Bejerano-Sagie, Michal [1 ]
Oppenheimer-Shaanan, Yaara [1 ]
Berlatzky, Idit [1 ]
Rouvinski, Alex [1 ]
Meyerovich, Mor [1 ]
Ben-Yehuda, Sigal [1 ]
机构
[1] Hebrew Univ Jerusalem, Fac Med, Dept Mol Biol, IL-91120 Jerusalem, Israel
基金
以色列科学基金会;
关键词
D O I
10.1016/j.cell.2006.03.039
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
In response to DNA damage, cells activate checkpoint signaling cascades to control cell-cycle progression and elicit DNA repair in order to maintain genomic integrity. The sensing and repair of lesions is critical for Bacillus subtilis cells entering the developmental process of sporulation as damaged DNA may prevent the cells from completing spore morphogenesis. We report the identification of the protein DisA (DNA integrity scanning protein, annotated YacK), which is required to delay the initiation of sporulation in response to chromosomal damage. DisA is a nonspecific DNA binding protein that forms a single focus, which moves rapidly within the bacterial cell, pausing at sites of DNA damage. We propose that the DisA focus scans along the chromosomes searching for lesions. Upon encountering a lesion, DisA delays entry into sporulation until the damage is repaired.
引用
收藏
页码:679 / 690
页数:12
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