S-box and T-box riboswitches and antisense RNA control a sulfur metabolic operon of Clostridium acetobutylicum

被引:101
作者
Andre, Gaelle [1 ]
Even, Sergine [1 ]
Putzer, Harald [2 ]
Burguiere, Pierre [1 ]
Croux, Christian [3 ]
Danchin, Antoine [1 ]
Martin-Verstraete, Isabelle [1 ]
Soutourina, Olga [1 ]
机构
[1] Inst Pasteur, CNRS, URA2171, F-75724 Paris 15, France
[2] Univ Paris 07, CNRS, UPR9073, Inst Phys & Chem Biol IBPC, F-75005 Paris, France
[3] UMR INSA INRA 792, UMR INSA CNRS 5504, Lab Biotechnol & Bioproc, F-31077 Toulouse 4, France
关键词
D O I
10.1093/nar/gkn601
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The ubiGmccBA operon of Clostridium acetobutylicum is involved in methionine to cysteine conversion. We showed that its expression is controlled by a complex regulatory system combining several RNA-based mechanisms. Two functional convergent promoters associated with transcriptional antitermination systems, a cysteine-specific T-box and an S-box riboswitch, are located upstream of and downstream from the ubiG operon, respectively. Several antisense RNAs were synthesized from the downstream S-box-dependent promoter, resulting in modulation of the level of ubiG transcript and of MccB activity. In contrast, the upstream T-box system did not appear to play a major role in regulation, leaving antisense transcription as the major regulatory mechanism for the ubiG operon. The abundance of sense and antisense transcripts was inversely correlated with the sulfur source availability. Deletion of the downstream promoter region completely abolished the sulfur-dependent control of the ubiG operon, and the expression of antisense transcripts in trans did not restore the regulation of the operon. Our data revealed important insights into the molecular mechanism of cis-antisense-mediated regulation, a control system only rarely observed in prokaryotes. We proposed a regulatory model in which the antisense RNA controlled the expression of the ubiG operon in cis via transcriptional interference at the ubiG locus.
引用
收藏
页码:5955 / 5969
页数:15
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