Stable chloroplast transformation in potato: use of green fluorescent protein as a plastid marker

被引：223

作者：

Sidorov, VA ^{[1
]}

Kasten, D ^{[1
]}

Pang, SZ ^{[1
]}

Hajdukiewicz, PTJ ^{[1
]}

Staub, JM ^{[1
]}

Nehra, NS ^{[1
]}

机构：

[1] Monsanto, St Louis, MO 63198 USA

来源：

PLANT JOURNAL | 1999年 / 19卷 / 02期

关键词：

D O I：

10.1046/j.1365-313X.1999.00508.x

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We describe here the development of a reproducible plastid transformation system for potato and regeneration of plants with uniformly transformed plastids. Two distinct tobacco-specific plastid vectors, pZS197 (Prrn/aadA/TpsbA) and pMON30125 (Prrn/GFP/Trps16::PpsbA/aadA/TpsbA), designed for integration into the large single copy and inverted repeat regions of the plastid genome, respectively, were bombarded into leaf explants of potato line FL1607. A total of three transgenic lines were selected out of 46 plates bombarded with pZS197 and three transgenic lines out of 104 plates were obtained with pMON30125. Development of a high frequency leaf-based regeneration system, a stringent selection scheme and optimization of biolistic transformation protocol were critical for recovery of plastid transformants. Plastid-expressed green fluorescent protein was used as a visual marker for identification of plastid transformants at the early stage of selection and shoot regeneration. The establishment of a plastid transformation system in potato, which has several advantages over routinely used nuclear transformation, offers new possibilities for genetic improvement of this crop.

引用

页码：209 / 216

页数：8

共 31 条

[1] WHY DO CHLOROPLASTS AND MITOCHONDRIA CONTAIN SO MANY COPIES OF THEIR GENOME [J].