Quantitative nucleic acids footprinting: Thermodynamic and kinetic approaches

被引：33

作者：

Petri, V ^{[1
]}

Brenowitz, M ^{[1
]}

机构：

[1] YESHIVA UNIV ALBERT EINSTEIN COLL MED, DEPT BIOCHEM, BRONX, NY 10461 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1997年 / 8卷 / 01期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/S0958-1669(97)80155-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Quantitative footprinting techniques allow a detailed analysis of the thermodynamic forces that characterize nucleic acid-ligand interactions and ligand-induced changes in nucleic acid structure by separately resolving the intrinsic and cooperative Gibbs free energy changes describing the reactions being investigated. A new implementation of the quantitative footprinting technique is the application of stopped-flow techniques to the study of kinetic reactions.

引用

页码：36 / 44

页数：9

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