Microtubule binding proteins CLIP-170, EB1, and p150Glued form distinct plus-end complexes

被引：41

作者：

Ligon, LA ^{[1
]}

Shelly, SS ^{[1
]}

Tokito, MK ^{[1
]}

Holzbaur, ELF ^{[1
]}

机构：

[1] Univ Penn, Dept Physiol, Philadelphia, PA 19104 USA

来源：

FEBS LETTERS | 2006年 / 580卷 / 05期

关键词：

microtubule-binding proteins; EBI; CLIP-170; p150(Glued);

D O I：

10.1016/j.febslet.2006.01.050

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Microtubule plus-end proteins CLIP-170 and EB1 dynamically track the tips of growing microtubules in vivo. Here we examine the association of these proteins with microtubules in vitro. CLIP-170 binds tubulin dimers and co-assembles into growing microtubules. EB1 binds tubulin dimers more weakly, so no co-assembly is observed. However, EB1 binds to CLIP170, and forms a co-complex with CLIP-170 and tubulin that is recruited to growing microtubule plus ends. The interaction between CLIP-170 and EB1 is competitively inhibited by the related CAP-Gly protein p150(Glued), which also localizes to microtubule plus ends in vivo. Based on these observations, we propose a model in which the formation of distinct plus-end complexes may differentially affect microtubule dynamics in vivo. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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页码：1327 / 1332

页数：6

相关论文

共 22 条

[1] Evidence that an interaction between EB1 and p150Glued is required for the formation and maintenance of a radial microtubule array anchored at the centrosome [J].

Askham, JM ;

Vaughan, KT ;

Goodson, HV ;

Morrison, EE .

MOLECULAR BIOLOGY OF THE CELL, 2002, 13 (10) :3627-3645

[2] Dynamic localization of CLIP-170 to microtubule plus ends is coupled to microtubule assembly [J].

Diamantopoulos, GS ;

Perez, F ;

Goodson, HV ;

Batelier, G ;

Melki, R ;

Kreis, TE ;

Rickard, JE .

JOURNAL OF CELL BIOLOGY, 1999, 144 (01) :99-112

[3] Structural basis for the activation of microtubule assembly by the EB1 and p150Glued complex [J].

Hayashi, I ;

Wilde, A ;

Mal, TK ;

Ikura, M .

MOLECULAR CELL, 2005, 19 (04) :449-460

[4] Crystal structure of the amino-terminal microtubule-binding domain of end-binding protein 1 (EB1) [J].

Hayashi, I ;

Ikura, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (38) :36430-36434

[5] Cytoplasmic linker proteins promote microtubule rescue in vivo [J].

Komarova, YA ;

Akhmanova, AS ;

Kojima, S ;

Galjart, N ;

Borisy, GG .

JOURNAL OF CELL BIOLOGY, 2002, 159 (04) :589-599

[6] Conformational changes in CLIP-170 regulate its binding to microtubules and dynactin localization [J].

Lansbergen, G ;

Komarova, Y ;

Modesti, M ;

Wyman, C ;

Hoogenraad, CC ;

Goodson, HV ;

Lemaitre, RP ;

Drechsel, DN ;

van Munster, E ;

Gadella, TWJ ;

Grosveld, F ;

Galjart, N ;

Borisy, GG ;

Akhmanova, A .

JOURNAL OF CELL BIOLOGY, 2004, 166 (07) :1003-1014

[7] Crystal structure of the cytoskeleton-associated protein glycine-rich (CAP-Gly) domain [J].

Li, SL ;

Finley, J ;

Liu, ZJ ;

Qiu, SH ;

Chen, HL ;

Luan, CH ;

Carson, M ;

Tsao, J ;

Johnson, D ;

Lin, GD ;

Zhao, J ;

Thomas, W ;

Nagy, LA ;

Sha, BD ;

DeLucas, LJ ;

Wang, BC ;

Luo, M .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2002, 277 (50) :48596-48601

[8] The microtubule plus-end proteins EB1 and dynactin have differential effects on microtubule polymerization [J].

Ligon, LA ;

Shelly, SS ;

Tokito, M ;

Holzbaur, ELF .

MOLECULAR BIOLOGY OF THE CELL, 2003, 14 (04) :1405-1417

[9] CLIP-170 family members: A motor-driven ride to microtubule plus ends [J].

Maekawa, H ;

Schiebel, E .

DEVELOPMENTAL CELL, 2004, 6 (06) :746-748

[10] MICROTUBULE DYNAMICS AND MICROTUBULE CAPS - A TIME-RESOLVED CRYOELECTRON MICROSCOPY STUDY [J].

MANDELKOW, EM ;

MANDELKOW, E ;

MILLIGAN, RA .

JOURNAL OF CELL BIOLOGY, 1991, 114 (05) :977-991