Chronoamperometric-based detection of hydrogen peroxide using palladium nanoparticles

Monodisperse palladium nanoparticles were used to develop a chronoamperometric-based sensor for the detection of hydrogen peroxide (H2O2). Thioether stabilised palladium nanoparticles with diameters of 1.8 nm, 2.0 nm and 3.0 nm were synthesised using a pyrolysis method. The palladium nanoparticles were immobilised on gold electrode surfaces using an organic linker. The effect of particle size on sensor performance was evaluated. The sensor was effective in detecting H2O2 in a Phosphate-Buffered Saline (PBS) solution and in milk samples. Detection limits of 45 mu M and 75 mu M, respectively, were obtained. The sensitivity of the sensor allowed detection of H2O2 at levels below the maximum admissible concentration.