Simple and rapid liquid chromatography method for determination of efavirenz in plasma

被引：49

作者：

Ramachandran, G

Kumar, AKH

Swaminathan, S ^{[1
]}

Venkatesan, P

Kumaraswami, V

Greenblatt, DJ

机构：

[1] Tuberculosis Res Ctr, Div HIV AIDS, Madras 600031, Tamil Nadu, India

[2] Tufts Univ, Sch Med, Dept Pharmacol & Expt Therapeut, Boston, MA USA

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2006年 / 835卷 / 1-2期

关键词：

efavirenz; plasma; HPLC;

D O I：

10.1016/j.jchromb.2006.03.014

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simple and rapid high performance liquid chromatographic method for determination of efavirenz in human plasma was developed. The method involved extraction of sample with ethyl acetate and analysis using a reversed-phase C-18 column (150 mm) with UV detection. The assay was linear from 0.0625 to 10.0 mu g/ml. The method was specific for efavirenz estimation and the drug was stable in plasma up to one month at -20 degrees C. The average recovery of efavirenz from plasma was 101%. Due to its simplicity, the assay can be used for pharmacokinetic studies and therapeutic drug monitoring of efavirenz. (c) 2006 Elsevier B.V. All rights reserved.

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页码：131 / 135

页数：5

相关论文

共 34 条

[1] Determination of twelve antiretroviral agents in human plasma sample using reversed-phase high-performance liquid chromatography [J].

Aymard, G ;

Legrand, M ;

Trichereau, N ;

Diquet, B .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2000, 744 (02) :227-240

[2] Therapeutic drug monitoring in HIV infection: current status and future directions [J].

Back, D ;

Gatti, G ;

Fletcher, C ;

Garaffo, R ;

Haubrich, R ;

Hoetelmans, R ;

Kurowski, M ;

Luber, A ;

Merry, C ;

Perno, CF .

AIDS, 2002, 16 :S5-S37

[3]

Barrett JS, 2002, INT J CLIN PHARM TH, V40, P507

[4]

BARTLETT J, 2000, P 7 C RETR OPP INF S

[5] Simultaneous determination of rifampicin and efavirenz in plasma [J].

Boffito, M ;

Tija, J ;

Reynolds, HE ;

Hoggard, PG ;

Bonora, S ;

Di Perri, G ;

Back, DJ .

THERAPEUTIC DRUG MONITORING, 2002, 24 (05) :670-674

[6] Determination of ethambutol in plasma by high-performance liquid chromatography after pre-column derivatization [J].

Chenevier, P ;

Massias, L ;

Gueylard, D ;

Farinotti, R .

JOURNAL OF CHROMATOGRAPHY B, 1998, 708 (1-2) :310-315

[7] Performance analysis of a rapid HPLC determination with the solvent demixing extraction of HIV antiproteases and efavirenz in plasma [J].

Cociglio, M ;

Hillaire-Buys, D ;

Peyrière, H ;

Alric, R .

JOURNAL OF CHROMATOGRAPHIC SCIENCE, 2003, 41 (02) :80-86

[8] Population pharmacokinetics and effects of efavirenz in patients with human immunodeficiency virus infection [J].

Csajka, C ;

Marzolini, C ;

Fattinger, K ;

Décosterd, LA ;

Fellay, J ;

Telenti, A ;

Biollaz, J ;

Buclin, T .

CLINICAL PHARMACOLOGY & THERAPEUTICS, 2003, 73 (01) :20-30

[9] Determination of atazanavir and other antiretroviral drugs (indinavir, amprenavir, nelfinavir and its active metabolite M8, saquinavir, ritonavir, lopinavir, nevirapine and efavirenz) plasma levels by high performance liquid chromatography with UV detection [J].

Dailly, E ;

Raffi, F ;

Jolliet, P .

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2004, 813 (1-2) :353-358

[10]

*DUP MERCK PHARM C, 1997, 226 DMP

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