Identification of a novel sonic hedgehog response element in the chicken ovalbumin upstream promoter-transcription factor II promoter

被引:51
作者
Krishnan, V [1 ]
Elberg, G [1 ]
Tsai, MJ [1 ]
Tsai, SY [1 ]
机构
[1] BAYLOR COLL MED,DEPT CELL BIOL,HOUSTON,TX 77030
关键词
AMINO-TERMINAL PRODUCT; MOTOR-NEURON INDUCTION; PROTEIN-KINASE-A; FLOOR PLATE; AUTOPROTEOLYTIC CLEAVAGE; ORPHAN RECEPTOR; MOUSE; GENE; EXPRESSION; FAMILY;
D O I
10.1210/me.11.10.1458
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Sonic hedgehog (Shh) is a secreted morphogen that regulates dorso-ventral patterning within the neural tube during embryonic development. it is well established that Shh can induce motor-neuron differentiation that coincides with the appearance of specific motor-neuron markers including chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) and IsIl. However, the mechanism of Shh-induced signaling pathway in vertebrates is not clearly defined. In this report we have identified COUP-TFII as a target gene for Shh. In addition we have used a 1.6-kb region of the COUP-TFII promoter to identify a target element that mediates the Shh-induced activity. Extensive deletions introduced within this region have further enabled us to identify a novel sonic hedgehog response element (ShhRE) in the COUP-TFII promoter. Point mutations introduced within the ShhRE reveal some key nucleotides that are essential for protein(s)-binding activity, Finally, the ShhRE is capable of functioning as a true enhancer element and can mediate Shh-induced transactivation of reporter gene via a heterologous promoter.
引用
收藏
页码:1458 / 1466
页数:9
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