Molecular approaches to the study of aquatic microbial communities

被引：15

作者：

MacGregor, BJ ^{[1
]}

机构：

[1] Northwestern Univ, Dept Civil Engn, Evanston, IL 60208 USA

来源：

CURRENT OPINION IN BIOTECHNOLOGY | 1999年 / 10卷 / 03期

关键词：

D O I：

10.1016/S0958-1669(99)80038-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Molecular methods for studying microbial communities are still under development. Enormous sequence catalogues can be collected; they must now be related to microbial activities. Messenger RNA detection, fluorescent in situ hybridization, cell sorting, and oligonucleotide array technology are currently being explored. Biases are associated with all these methods, but combined approaches offer checks and balances.

引用

页码：220 / 224

页数：5

共 59 条

[1] In situ methods for assessment of microorganisms and their activities [J].

Amann, R ;

Kuhl, M .

CURRENT OPINION IN MICROBIOLOGY, 1998, 1 (03) :352-358

[2] Monitoring the community structure of wastewater treatment plants: a comparison of old and new techniques [J].

Amann, R ;

Lemmer, H ;

Wagner, M .

FEMS MICROBIOLOGY ECOLOGY, 1998, 25 (03) :205-215

[3] PHYLOGENETIC IDENTIFICATION AND IN-SITU DETECTION OF INDIVIDUAL MICROBIAL-CELLS WITHOUT CULTIVATION [J].

AMANN, RI ;

LUDWIG, W ;

SCHLEIFER, KH .

MICROBIOLOGICAL REVIEWS, 1995, 59 (01) :143-169

[4]

Binder BJ, 1998, APPL ENVIRON MICROB, V64, P3346

[5] Direct linking of microbial populations to specific biogeochemical processes by 13C-labelling of biomarkers [J].

Boschker, HTS ;

Nold, SC ;

Wellsbury, P ;

Bos, D ;

de Graaf, W ;

Pel, R ;

Parkes, RJ ;

Cappenberg, TE .

NATURE, 1998, 392 (6678) :801-805

[6] Modelling carbon responses of tundra ecosystems to historical and projected climate: sensitivity of pan-Arctic carbon storage to temporal and spatial variation in climate [J].

McGuire, AD ;

Clein, JS ;

Melillo, JM ;

Kicklighter, DW ;

Meier, RA ;

Vorosmarty, CJ ;

Serreze, MC .

GLOBAL CHANGE BIOLOGY, 2000, 6 :141-159

[7]

Brinkhoff T, 1998, APPL ENVIRON MICROB, V64, P4650

[8] In situ reverse transcription, an approach to characterize genetic diversity and activities of prokaryotes [J].

Chen, F ;

Gonzalez, JM ;

Dustman, WA ;

Moran, MA ;

Hodson, RE .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1997, 63 (12) :4907-4913

[9] Genetic diversity in marine algal virus communities as revealed by sequence analysis of DNA polymerase genes [J].

Chen, F ;

Suttle, CA ;

Short, SM .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (08) :2869-2874

[10] Bacterial transcript imaging by hybridization of total RNA to oligonucleotide arrays [J].

de Saizieu, A ;

Certa, U ;

Warrington, J ;

Gray, C ;

Keck, W ;

Mous, J .

NATURE BIOTECHNOLOGY, 1998, 16 (01) :45-48

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