Visualization of synaptic specializations in live C-elegans with synaptic vesicle protein-GFP fusions

被引：178

作者：

Nonet, ML ^{[1
]}

机构：

[1] Washington Univ, Sch Med, Dept Anat & Neurobiol, St Louis, MO 63110 USA

来源：

JOURNAL OF NEUROSCIENCE METHODS | 1999年 / 89卷 / 01期

关键词：

in vivo imaging; fluorescence microscopy; green fluorescent protein; synapse; synaptic vesicle; synaptobrevin; synaptogyrin; AP180;

D O I：

10.1016/S0165-0270(99)00031-X

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Synaptic specializations are difficult to visualize at the light microscope level in living preparations. To circumvent this problem, synaptic vesicle proteins were fused to green fluorescent protein (GFP) and expressed in C. elegans neurons. C. elegans synaptobrevin-GFP and synaptogyrin-GFP fusion proteins were observed to target to synaptic sites. This localization allowed the visualization of synaptic specializations in living animals with light microscopy. Restricted expression of synaptobrevin-GFP fusions in subsets of neurons enables the visualization of individual presynaptic varicosities. The cell biology underlying the sorting of synaptic vesicle proteins, trafficking of vesicles to terminals, and the development of presynaptic specializations is now more amenable to forward genetic analysis using these synaptic markers. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：33 / 40

页数：8

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