The effect of fibrin stabilizing factor (F.XIII) on healing of bone defects in normal and uncontrolled diabetic rats

This study was undertaken to examine the effect of fibrin stabilizing factor (EXIII) on healing of bone defects in normal and uncontrolled diabetic rats. Eighty rats were divided into two groups: group I (diabetic) and group Il (non-diabetic) (40 rats each). Diabetes was induced in group I using streptozotocin. Both groups were divided into two subgroups, control and experimental (20 rats each). Bone defect was created in the mandible. Bats in the experimental subgroups were injected with EXIII, while those of the control groups were injected with saline (EXIII solvent). Animals were killed at varying intervals and tissue sections stained with hematoxyline and eosin and Van-Gieson stains were examined. Differences in collagen deposition and bone formation were compared in both control and experimental groups. Collagen deposition was evident and appeared more oriented in diabetic rats treated with EXIII, and signs of bone deposition started in the experimental group earlier than in the control group. On the other hand, EXIII did not significantly affect healing in non-diabetic rats. It is concluded from these results that EXIII may enhance early stages of bone healing in uncontrolled diabetic rats.