Morphine tolerance and transcription factor expression in mouse spinal cord tissue

被引：31

作者：

Li, XQ

Clark, JD

机构：

[1] Stanford Univ, Dept Anesthesiol, Palo Alto, CA 94304 USA

[2] Vet Adm Palo Alto Hlth Care Syst, Palo Alto, CA 94304 USA

来源：

NEUROSCIENCE LETTERS | 1999年 / 272卷 / 02期

关键词：

c-Fos; Fos B; Delta Fos B; CREB; morphine; tolerance; spinal cord;

D O I：

10.1016/S0304-3940(99)00559-5

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Little is known about changes in gene expression responsible for the acquisition and maintenance of tolerance to the analgesic effects of opioids. In these studies we examine changes in the expression of several transcription factors in the spinal cords of morphine tolerant C57BL/6 mice. Western blots demonstrate a 1.9-fold increase in cyclic AMP response element binding protein (CREB) and a 2.4-fold increase phospho-CREB immunoreactivities in spinal cord homogenates from morphine tolerant animals. Likewise, Fos B and Delta Fos B immunoreactivities were increased 2.2 and 2.3-fold, respectively. The expression of c-Fos remained unchanged. Immunohistological analysis showed the increase of phospho-CREB and Fos B/Delta Fos B to be primarily in the dorsal horn region of the spinal cord. We conclude that chronic exposure to opioids causes changes in gene expression in sensory processing areas of the spinal cord. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：79 / 82

页数：4

共 20 条

[1] Competitive and non-competitive NMDA antagonists block the development of antinociceptive tolerance to morphine, but not to selective mu or delta opioid agonists in mice [J].