CULTURE VERSUS POLYMERASE CHAIN REACTION FOR THE ETIOLOGIC DIAGNOSIS OF COMMUNITY-ACQUIRED PNEUMONIA IN ANTIBIOTIC-PRETREATED PEDIATRIC PATIENTS

被引：21

作者：

Deng, Jikui ^{[1
,2
]}

Zheng, Yuejie ^{[1
]}

Zhao, Ruizhen ^{[1
]}

Wright, Peter F. ^{[3
,4
]}

Stratton, Charles W. ^{[2
,4
]}

Tang, Yi-Wei ^{[2
,4
]}

机构：

[1] China Med Univ, Shenzhen Childrens Hosp, Shenzhen, Peoples R China

[2] Vanderbilt Univ, Med Ctr, Dept Med, Nashville, TN USA

[3] Vanderbilt Univ, Med Ctr, Dept Pediat, Nashville, TN 37232 USA

[4] Vanderbilt Univ, Med Ctr, Dept Pathol, Nashville, TN 37232 USA

来源：

PEDIATRIC INFECTIOUS DISEASE JOURNAL | 2009年 / 28卷 / 01期

关键词：

polymerase chain reaction; community-acquired pneumonia; etiology; STREPTOCOCCUS-PNEUMONIAE; HOSPITALIZED CHILDREN; RESPIRATORY-INFECTION; ATYPICAL PATHOGENS; PCR; ASPIRATION; SAMPLES; NEEDLE;

D O I：

10.1097/INF.0b013e3181817bd2

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Tracheal aspirates were collected from 176 hospitalized antibiotic-pretreated children with community-acquired pneumonia. Haemophilus influenzae and Streptococcus pneumoniae were detected by both culture and target-enriched multiplex (TEM)-PCR whereas Mycoplasma pneumoniae and Chlamydia pneumoniae were detected by TEM-PCR Only. TEM-PCR detected more S. pneumoniae (32 vs. 7) and H. influenzae (29 vs. 23) than did culture. TEM-PCR detected an additional 26 M. pneumoniae and 1 C pneumoniae. TEM-PCR significantly enhances the pathogen-specific diagnosis of CAP in children.

引用

页码：53 / 55

页数：3

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