共 31 条
A bacterial inhibitor of host programmed cell death defenses is an E3 ubiquitin ligase
被引:275
作者:
Janjusevic, R
Abramovitch, RB
Martin, GB
Stebbins, CE
机构:
[1] Cornell Univ, Boyce Thompson Inst Plant Res, Ithaca, NY 14853 USA
[2] Rockefeller Univ, Lab Struct Microbiol, New York, NY 10021 USA
[3] Cornell Univ, Dept Plant Pathol, Ithaca, NY 14853 USA
来源:
关键词:
D O I:
10.1126/science.1120131
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
The Pseudomonas syringae protein AvrPtoB is translocated into plant cells, where it inhibits immunity-associated programmed cell. death (PCD). The structure of a C-terminal domain of AvrPtoB that is essential for anti-PCD activity reveals an unexpected homology to the U-box and RING-finger components of eukaryotic E3 ubiquitin ligases, and we show that AvrPtoB has ubiquitin ligase activity. Mutation of conserved residues involved in the binding of E2 ubiquitin-conjugating enzymes abolishes this activity in vitro, as well as anti-PCD activity in tomato leaves, which dramatically decreases virulence. These results show that Pseudomonas syringae uses a mimic of host E3 ubiquitin ligases to inactivate plant defenses.
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页码:222 / 226
页数:5
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