Stabilization and enhanced reactivity of actinorhodin polyketide synthase minimal complex in polymer-nucleotide coacervate droplets

被引：89

作者：

Crosby, John ^{[1
]}

Treadwell, Tom ^{[1
]}

Hammerton, Michelle ^{[1
]}

Vasilakis, Konstantinos ^{[1
]}

Crump, Matthew P. ^{[1
]}

Williams, David S. ^{[1
]}

Mann, Stephen ^{[1
]}

机构：

[1] Univ Bristol, Sch Chem, Bristol BS8 1TS, Avon, England

来源：

CHEMICAL COMMUNICATIONS | 2012年 / 48卷 / 97期

关键词：

ACYL CARRIER PROTEIN; AROMATIC POLYKETIDES; BIOSYNTHESIS;

D O I：

10.1039/c2cc36533b

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Compartmentalization of the minimal complex of actinorhodin polyketide synthase in coacervate liquid droplets produces enhanced yields of shunt polyketides under conditions of low and high ionic strength.

引用

页码：11832 / 11834

页数：3

共 23 条

[1] FUNCTIONAL-PROPERTIES OF HEMOGLOBIN IMMOBILIZED IN COACERVATES PREPARED FROM GELATIN-A AND POLYANIONIC CARBOHYDRATES [J].

BROUWER, M ;

CASHON, R ;

BONAVENTURA, J .

BIOTECHNOLOGY AND BIOENGINEERING, 1990, 35 (08) :831-836

[2] β-Glucuronidase activity following complex coacervation and spray drying microencapsulation [J].

Burgess, DJ ;

Ponsart, S .

JOURNAL OF MICROENCAPSULATION, 1998, 15 (05) :569-579

[3] Efficient synthesis of aromatic polyketides in vitro by the actinorhodin polyketide synthase [J].

Carreras, CW ;

Pieper, R ;

Khosla, C .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1996, 118 (21) :5158-5159

[4] The structural role of the carrier protein - active controller or passive carrier [J].

Crosby, John ;

Crump, Matthew P. .

NATURAL PRODUCT REPORTS, 2012, 29 (10) :1111-1137

[5] Solution structure of the actinorhodin polyketide synthase acyl carrier protein from Streptomyces coelicolor A3(2) [J].

Crump, MP ;

Crosby, J ;

Dempsey, CE ;

Parkinson, JA ;

Murray, M ;

Hopwood, DA ;

Simpson, TJ .

BIOCHEMISTRY, 1997, 36 (20) :6000-6008

[6] OVERPRODUCTION OF THE ACYL CARRIER PROTEIN-COMPONENT OF A TYPE-II POLYKETIDE SYNTHASE STIMULATES PRODUCTION OF TETRACENOMYCIN BIOSYNTHETIC INTERMEDIATES IN STREPTOMYCES-GLAUCESCENS [J].

DECKER, H ;

SUMMERS, RG ;

HUTCHINSON, CR .

JOURNAL OF ANTIBIOTICS, 1994, 47 (01) :54-63

[7] Designs for life: protocell models in the laboratory [J].

Dzieciol, Alicja J. ;

Mann, Stephen .

CHEMICAL SOCIETY REVIEWS, 2012, 41 (01) :79-85

[8]

EVREINOVA TN, 1955, DOKL AKAD NAUK SSSR+, V105, P137

[9]

Fu H, 1994, Chem Biol, V1, P205, DOI 10.1016/1074-5521(94)90012-4

[10] ENGINEERED BIOSYNTHESIS OF NOVEL POLYKETIDES - DISSECTION OF THE CATALYTIC SPECIFICITY OF THE ACT KETOREDUCTASE [J].

FU, H ;

EBERTKHOSLA, S ;

HOPWOOD, DA ;

KHOSLA, C .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1994, 116 (10) :4166-4170

← 1 2 3 →