Biochemical study of idebenone effect on mitochondrial metabolism of yeast

被引：6

作者：

Chapela, Sebastian P. ^{[1
]}

Burgos, Hilda I. ^{[1
]}

Salazar, Ana I. ^{[1
]}

Nievas, Ines ^{[1
]}

Kriguer, Norberto ^{[1
]}

Stella, Carlos A. ^{[1
]}

机构：

[1] Univ Buenos Aires, Fac Med, Dept Bioquim Humana, RA-2155 Paraguari, Argentina

来源：

CELL BIOLOGY INTERNATIONAL | 2008年 / 32卷 / 01期

关键词：

Idebenone; Saccharomyces cerevisiae; Mitochondrial potential;

D O I：

10.1016/j.cellbi.2007.08.003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The aim of this work was to study the effect of the drug idebenone on the growth of a strain of Saccharomyces cerevisiae yeast and its respiratory-deficient mutant (rho(0)). We took this yeast as a model system of the interaction of the drug with mammalian cells. The effect of idebenone was evaluated in rich and minimal media. In the S288c strain, idebenone exerted a growth inhibitory effect in concentrations higher than 50 mu M in media containing a carbon source consumed at mitochondrial level. In conditions of low oxygen supply, idebenone allows yeast to keep a cellular yielding comparable with conditions of normal oxygen supply. Also, the presence of idebenone in the growth media increased by 50% the fluorescence signal of rhodamine 123, indicating a higher mitochondrial membrane potential. The results could explain the effect of idebenone in the treatment of diseases in which oxygen deficiency alters the energetic metabolism of the cell. (C) 2007 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.

引用

页码：146 / 150

页数：5

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