Extended longevity of Caenorhabditis elegans by knocking in extra copies of hsp70F, a homolog of mot-2 (mortalin)/mthsp70/Grp75

The Caenorhabditis elegans homolog of mortalin/mthsp70/Grp75; (called mot-2 hereafter) was isolated by screening of a nematode cDNA library with mouse mot-2 cDNA. The isolated clone matched to hsp70F of C elegans. Analysis with two of the antibodies raised against hsp70F revealed that unlike mammalian mot-2, it is heat inducible. Transient induction of hsp70F by heat shock led to a slight (< 13%) extension in the C elegans life span. The transgenic worms that constitutively over-expressed hsp70F predominantly in muscle showed life span extension (similar to 43% for mean and similar to 45% for maximum life span) as compared to the wild-type and green fluorescent protein-transgenic worms. Life span extension of human cells was obtained by over-expression of mot-2 [Kaul et al. (2000) FEBS Lett. 474, 159-164]. Our results show, for the first time, that this member of the hsp70 family governs the longevity of worms and thus there are common pathways that determine mammalian and worm longevity. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.