The Vibrio cholerae chitin utilization program

被引:418
作者
Meibom, KL
Li, XBB
Nielsen, AT
Wu, CY
Roseman, S
Schoolnik, GK [1 ]
机构
[1] Stanford Univ, Sch Med, Dept Med, Div Infect Dis & Geog Med, Stanford, CA 94305 USA
[2] Stanford Univ, Sch Med, Dept Microbiol & Immunol, Stanford, CA 94305 USA
[3] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA
[4] Johns Hopkins Univ, McCollum Pratt Inst, Baltimore, MD 21218 USA
关键词
D O I
10.1073/pnas.0308707101
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Chitin, an insoluble polymer of GIcNAc, is an abundant source of carbon, nitrogen, and energy for marine microorganisms. Microarray expression profiling and mutational studies of Vibrio cholerae growing on a natural chitin surface, or with the soluble chitin oligosaccharides (GIcNAc)(2-6), GIcNAc, or the glucosamine dimer (GIcN)(2) identified three sets of differentially regulated genes. We show that (i) ChiS, a sensor histidine kinase, regulates expression of the (GIcNAc)(2-6) gene set, including a (GIcNAc)(2) catabolic operon, two extracellular chitinases, a chitoporin, and a PiIA-containing type IV pilus, designated ChiRP (chitin-regulated pilus) that confers a significant growth advantage to V. cholerae on a chitin surface; (ii) GIcNAc causes the coordinate expression of genes involved with chitin chemotaxis and adherence and with the transport and assimilation of GIcNAc; (iii) (GIcN)(2) induces genes required for the transport and catabolism of nonacetylated chitin residues; and (iv) the constitutively expressed MSHA pilus facilitates adhesion to the chitin surface independent of surface chemistry. Collectively, these results provide a global portrait of a complex, multistage V. cholerae program for the efficient utilization of chitin.
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收藏
页码:2524 / 2529
页数:6
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