Human fibroblasts in vitro senesce with a donor-specific telomere length

被引：19

作者：

Serra, V

von Zglinicki, T ^{[1
]}

机构：

[1] Humboldt Univ, Inst Pathol, Berlin, Germany

[2] Newcastle Univ, Wolfson Res Ctr, Gen Hosp, Dept Gerontol, Newcastle Upon Tyne NE4 6BE, Tyne & Wear, England

来源：

FEBS LETTERS | 2002年 / 516卷 / 1-3期

基金：

英国医学研究理事会;

关键词：

fibroblast; senescence; hayflick limit; telomere;

D O I：

10.1016/S0014-5793(02)02504-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In human fibroblasts, replicative senescence, telomere length and donor age are closely interrelated. We analyzed these relationships for fibroblast strains from 14 healthy human donors in the age range of 28-90 years. In vitro replicative capacity was correlated more closely with donor age than with telomere length ex vivo, especially for healthy donors. Telomere length at senescence was as variable as at cell explantation and increased with donor age. The data suggest a donor-specific, age-dependent regulation of the telomere length threshold that triggers senescence in human fibroblasts. (C) 2002 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

收藏

页码：71 / 74

页数：4

相关论文

共 21 条

[1] TELOMERE LENGTH PREDICTS REPLICATIVE CAPACITY OF HUMAN FIBROBLASTS [J].

ALLSOPP, RC ;

VAZIRI, H ;

PATTERSON, C ;

GOLDSTEIN, S ;

YOUNGLAI, EV ;

FUTCHER, AB ;

GREIDER, CW ;

HARLEY, CB .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (21) :10114-10118

[2] From cells to organisms: can we learn about aging from cells in culture? [J].

Campisi, J .

EXPERIMENTAL GERONTOLOGY, 2001, 36 (4-6) :607-618

[3] Relationship between donor age and the replicative lifespan of human cells in culture: A reevaluation [J].

Cristofalo, VJ ;

Allen, RG ;

Pignolo, RJ ;

Martin, BG ;

Beck, JC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1998, 95 (18) :10614-10619

[4]

Figueroa R, 2000, CANCER RES, V60, P2770

[5] Mammalian telomeres end in a large duplex loop [J].

Griffith, JD ;

Comeau, L ;

Rosenfield, S ;

Stansel, RM ;

Bianchi, A ;

Moss, H ;

de Lange, T .

CELL, 1999, 97 (04) :503-514

[6] The illusion of cell immortality [J].

Hayflick, L .

BRITISH JOURNAL OF CANCER, 2000, 83 (07) :841-846

[7] SERIAL CULTIVATION OF HUMAN DIPLOID CELL STRAINS [J].

HAYFLICK, L ;

MOORHEAD, PS .

EXPERIMENTAL CELL RESEARCH, 1961, 25 (03) :585-+

[8] The shortest telomere, not average telomere length, is critical for cell viability and chromosome stability [J].

Hemann, MT ;

Strong, MA ;

Hao, LY ;

Greider, CW .

CELL, 2001, 107 (01) :67-77

[9] BJ fibroblasts display high antioxidant capacity and slow telomere shortening independent of hTERT transfection [J].

Lorenz, M ;

Saretzki, G ;

Sitte, N ;

Metzkow, S ;

von Zglinicki, T .

FREE RADICAL BIOLOGY AND MEDICINE, 2001, 31 (06) :824-831

[10] Accumulation of short telomeres in human fibroblasts prior to replicative senescence [J].

Martens, UM ;

Chavez, EA ;

Poon, SSS ;

Schmoor, C ;

Landsdorp, PM .

EXPERIMENTAL CELL RESEARCH, 2000, 256 (01) :291-299