Structural studies of water-soluble polysaccharides of an edible mushroom, Termitomyces eurhizus.: A reinvestigation

被引：95

作者：

Mondal, S ^{[1
]}

Chakraborty, I ^{[1
]}

Pramanik, M ^{[1
]}

Rout, D ^{[1
]}

Islam, SS ^{[1
]}

机构：

[1] Vidyasagar Univ, Dept Chem & Chem Technol, W Midnapore 721102, W Bengal, India

来源：

CARBOHYDRATE RESEARCH | 2004年 / 339卷 / 06期

关键词：

mushroom polysaccharide; Termitomyces eurhizus; structure; NMR analysis;

D O I：

10.1016/j.carres.2004.02.019

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The structure of two polysaccharides isolated from the hot aqueous extract of fruiting bodies of the mushroom, Termitomyces eurhizus, have been reinvestigated. These consist of two homogeneous fractions PS-I and PS-II. PS-I contains only D-glucose as the monosaccharide constituent. From methylation analysis and periodate oxidation studies, followed by GLC-MS analysis the linkages, the sugar units in PS-I were identified as (1 --> 3)-D-Glcp and (1 --> 6)-D-Glcp. PS-II contains D-glucose, and the mode of linkage Of D-glucose was identified as (1 --> 6)-D)-Glcp. Finally, the following possible structures of the polysaccharides were assigned using H-1. 2D-COSY, TOCSY, NOESY and C-13 NMR spectral analysis: [--> 6)-alpha-D-Glcp-(1]m--> [3)-alpha-D-Glcp-(l]n --> m:n = 2.5:1 PS-I (alpha-Glucan) [-->6)-alpha-D-Glcp-(1--> 6)-alpha-D-Glcp-(1-->]n PS-II (alpha-Glucan) (C) 2004 Elsevier Ltd. All rights reserved.

引用

页码：1135 / 1140

页数：6

共 25 条

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NMR-SPECTROSCOPY IN THE STRUCTURAL ELUCIDATION OF OLIGOSACCHARIDES AND GLYCOSIDES [J].