Rapid genotypic differentiation of Lactococcus lactis subspecies and biovar

被引：51

作者：

Beimfohr, C ^{[1
]}

Ludwig, W ^{[1
]}

Schleifer, KH ^{[1
]}

机构：

[1] TECH UNIV MUNICH,LEHRSTUHL MIKROBIOL,D-80290 MUNICH,GERMANY

来源：

SYSTEMATIC AND APPLIED MICROBIOLOGY | 1997年 / 20卷 / 02期

关键词：

Lactococcus lactis; PCR approach; histidine biosynthesis operon; genotypic subspecies differentiation;

D O I：

10.1016/S0723-2020(97)80068-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A highly efficient, rapid and reliable PCR-based method for distinguishing the subspecies and biovar of Lactococcus lactis (Lactococcus lactis subsp. lactis, L. lactis subsp. lactis biovar diacetylactis and L. lactis subsp. cremoris) is described. Primers complementary to positions in the histidine biosynthesis operon with distinct specificities have been constructed. PCR analysis with extracted DNA or with cells of different Lactococcus lactis strains resulted in specific fragments which could be visualized after agarose gel electrophoresis. The length polymorphisms of the PCR fragments allowed a clear distinction of the L. lactis subspecies and biovar. In our study we have also included strains which in contrast to their 16S rRNA sequence belong to a different subspecies as determined by their phenotype. The results of the PCR assay agree perfectly well with the identification based on the 16S rRNA analysis.

引用

页码：216 / 221

页数：6

共 14 条

[1]

BEIMFOHR C, 1993, SYST APPL MICROBIOL, V16, P450, DOI [10.1016/S0723-2020(11)80279-1, 10.1078/072320202320771525]

[2] GENE ORGANIZATION AND PRIMARY STRUCTURE OF A RIBOSOMAL-RNA OPERON FROM ESCHERICHIA-COLI [J].

BROSIUS, J ;

DULL, TJ ;

SLEETER, DD ;

NOLLER, HF .

JOURNAL OF MOLECULAR BIOLOGY, 1981, 148 (02) :107-127

[3]

DE MAN J. C., 1960, JOUR APPL BACT, V23, P130, DOI 10.1111/j.1365-2672.1960.tb00188.x

[4] MOSAIC STRUCTURE OF LARGE REGIONS OF THE LACTOCOCCUS-LACTIS SUBSP CREMORIS CHROMOSOME [J].

DELORME, C ;

GODON, JJ ;

EHRLICH, SD ;

RENAULT, P .

MICROBIOLOGY-SGM, 1994, 140 :3053-3060

[5] DIVERGENCE OF GENOMIC SEQUENCES BETWEEN LACTOCOCCUS-LACTIS SUBSP LACTIS AND LACTOCOCCUS-LACTIS SUBSP CREMORIS [J].

GODON, JJ ;

DELORME, C ;

EHRLICH, SD ;

RENAULT, P .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1992, 58 (12) :4045-4047

[6] INTRODUCTION OF SILENT MUTATIONS IN A PROTEINASE GENE OF LACTOCOCCUS-LACTIS AS A USEFUL MARKER FOR MONITORING STUDIES [J].

HERTEL, C ;

LUDWIG, W ;

SCHLEIFER, KH .

SYSTEMATIC AND APPLIED MICROBIOLOGY, 1992, 15 (03) :447-452

[7] DETECTION AND CHARACTERIZATION OF LACTOSE-UTILIZING LACTOCOCCUS SPP IN NATURAL ECOSYSTEMS [J].

KLIJN, N ;

WEERKAMP, AH ;

DEVOS, WM .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1995, 61 (02) :788-792

[8] IDENTIFICATION OF MESOPHILIC LACTIC-ACID BACTERIA BY USING POLYMERASE CHAIN REACTION-AMPLIFIED VARIABLE REGIONS OF 16S RIBOSOMAL-RNA AND SPECIFIC DNA PROBES [J].

KLIJN, N ;

WEERKAMP, AH ;

DEVOS, WM .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (11) :3390-3393

[9] PROCEDURE FOR ISOLATION OF DEOXYRIBONUCLEIC ACID FROM MICRO-ORGANISMS [J].

MARMUR, J .

JOURNAL OF MOLECULAR BIOLOGY, 1961, 3 (02) :208-&

[10] DEVELOPMENT AND APPLICATION OF OLIGONUCLEOTIDE PROBES FOR IDENTIFICATION OF LACTOCOCCUS-LACTIS SUBSP CREMORIS [J].

SALAMA, M ;

SANDINE, W ;

GIOVANNONI, S .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1991, 57 (05) :1313-1318

← 1 2 →