Identification of a high-molecular-weight cadmium-binding protein in copper-resistant Bacillus acidocaldarius cells

被引：10

作者：

Capasso, C ^{[1
]}

Nazzaro, F ^{[1
]}

Marulli, F ^{[1
]}

Capasso, A ^{[1
]}

LaCara, F ^{[1
]}

Parisi, E ^{[1
]}

机构：

[1] CNR,INST PROT BIOCHEM & ENZYMOL,I-80125 NAPLES,ITALY

来源：

RESEARCH IN MICROBIOLOGY | 1996年 / 147卷 / 04期

关键词：

copper; cadmium; metal-binding proteins; Bacillus acidocaldarius; heavy metal resistance; metal toxicity;

D O I：

10.1016/0923-2508(96)81389-1

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacillus acidocaldarius grown in the presence of Cu++ was capable of accumulating the metal in the form of a protease-sensitive high molecular weight (HMW) moiety whose formation was inhibited by actinomycin D. Only cells preadapted in Cu++ were able to grow in a Cd++ -containing medium. A cell-free extract from cadmium-stressed cells was fractionated by gel-permeation chromatography. The majority of cadmium was found associated with a HMW protein fraction which was further purified by anion exchange chromatography and high-performance liquid chromatography. The molecular weight of the purified protein was estimated to be 23,000 by SDS-PAGE. Amino acid analysis showed a low cysteine content and an abundance of aspartate and glutamate. It is likely that the cadmium-binding protein is an essential component of the mechanism mediating recovery from heavy metal toxicity.

引用

页码：287 / 296

页数：10

共 37 条

[1] INORGANIC-PHOSPHATE ACCUMULATION AND CADMIUM DETOXIFICATION IN KLEBSIELLA-AEROGENES NCTC-418 GROWING IN CONTINUOUS CULTURE [J].