Functional annotation of a full-length Arabidopsis cDNA collection

被引：509

作者：

Seki, M

Narusaka, M

Kamiya, A

Ishida, J

Satou, M

Sakurai, T

Nakajima, M

Enju, A

Akiyama, K

Oono, Y

Muramatsu, M

Hayashizaki, Y

Kawai, J

Carninci, P

Itoh, M

Ishii, Y

Arakawa, T

Shibata, K

Shinagawa, A

Shinozaki, K

机构：

[1] RIKEN, Tsukuba Inst, Plant Mol Biol Lab, Tsukuba, Ibaraki 3050074, Japan

[2] RIKEN, Genom Sci Ctr, Plant Funct Genom Res Grp, Plant Mutat Explorat Team, Tsukuba, Ibaraki 3050074, Japan

[3] Univ Tsukuba, Masters Program Biosyst Studies, Tsukuba, Ibaraki 3050074, Japan

[4] RIKEN GSC, Yokohama Inst, Lab Genome Explorat Res Grp, Tsurumi Ku, Yokohama, Kanagawa 2300045, Japan

[5] RIKEN, Genome Sci Lab, Wako, Saitama 3510198, Japan

来源：

SCIENCE | 2002年 / 296卷 / 5565期

关键词：

D O I：

10.1126/science.1071006

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Full-length complementary DNAs (cDNAs) are essential for the correct annotation of genomic sequences and for the functional analysis of genes and their products. We isolated 155,144 RIKEN Arabidopsis full-length (RAFL) cDNA clones. The 3'-end expressed sequence tags (ESTs) of 155,144 RAFL cDNAs were clustered into 14,668 nonredundant cDNA groups, about 60% of predicted genes. We also obtained 5' ESTs from 14,034 nonredundant cDNA groups and constructed a promoter database. The sequence database of the RAFL cDNAs is useful for promoter analysis and correct annotation of predicted transcription units and gene products. Furthermore, the full-length cDNAs are useful resources for analyses of the expression profiles, functions, and structures of plant proteins.

引用

页码：141 / 145

页数：5

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