Characterization of the biotin biosynthesis pathway in Saccharomyces cerevisiae and evidence for a cluster containing BI05, a novel gene involved in vitamer uptake

被引：64

作者：

Phalip, V

Kuhn, I

Lemoine, Y

Jeltsch, JM

机构：

[1] Ecole Super Biotechnol Strasbourg, Biotechnol Lab, F-67400 Illkirch Graffenstaden, France

[2] Inst Pasteur, F-59019 Lille, France

来源：

GENE | 1999年 / 232卷 / 01期

关键词：

gene cluster; membrane protein; mutant isolation; phenotypic complementation;

D O I：

10.1016/S0378-1119(99)00117-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

An engineered mutant of Saccharomyces cerevisiae affected in biotin biosynthesis has been isolated. This mutant allowed the characterization of a bio cluster (BIO3-4-5). We demonstrate that BIO3 (YNR058w) and BIO4 (YNR057c) encode, respectively, a 7,8-diaminopelargonic acid aminotransferase and a dethiobiotin synthase, involved in the biotin biosynthesis pathway. A novel gene, BIO5 (YNR056c), is present immediately downstream from BIO4. This gene encodes Bio5p, a protein with 11 putative transmembrane regions. Uptake experiments performed with labeled 7-keto 8-aminopelargonic acid indicate that Bio5p is responsible for transport into the cell of 7-keto 8-aminopelargonic acid. (C) 1999 Elsevier Science B.V. All rights reserved.

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收藏

页码：43 / 51

页数：9

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