Mechanism of DNA replication in eukaryotic cells: cellular host factors stimulating adenovirus DNA replication

被引：62

作者：

de Jong, RN

van der Vliet, PC

机构：

[1] Univ Utrecht, Physiol Chem Lab, NL-3521 GG Utrecht, Netherlands

[2] Univ Utrecht, Ctr Biomed Genet, NL-3521 GG Utrecht, Netherlands

来源：

GENE | 1999年 / 236卷 / 01期

关键词：

DNA polymerase; DNA synthesis; nuclear factor I; Oct-1; transcription factor;

D O I：

10.1016/S0378-1119(99)00249-8

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Replication of adenovirus (Ad) DNA depends on interactions between three viral and three cellular proteins. Human transcription factors NFI and Oct-1 recruit the Ad DNA polymerase to the origin of DNA replication as a complex with the Ad protein primer pTP. High affinity and specificity DNA binding to recognition sites in this origin by the transcription factors stimulate and stabilize pre-initiation complex formation to compensate for the low binding specificity of the pTP/pol complex. In this review, we discuss the properties of NFI and Oct-1 and the mechanism by which they enhance initiation of DNA replication. We propose a model that describes the dynamics of initiation and elongation as well as the assembly and disassembly of the preinitiation complex; (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：1 / 12

页数：12

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